rd10 mice  (Inserm Transfert)

Journal: Cell Communication and Signaling : CCS

Article Title: Interleukin 27-induced photoreceptor survival is associated with suppression of a novel Muller glia subpopulation

doi: 10.1186/s12964-026-02885-1

Figure Lengend Snippet: Delivery of IL-27 early in disease leads to sustained photoreceptor rescue measured four weeks after intravitreal injection. Mice were injected at P18 and phenotypes were analyzed at P45. A IL-27 increases visual acuity at P45 compared to saline and untreated (UT) rd10 mice, indicating a delay in vision loss following injection. Only the responses of the left eyes are shown (injected with IL-27 or saline, or uninjected for the untreated mice). ** p =0.0003, IL-27 vs. UT; # p =0.0121, IL-27 vs. saline; * p =0.039, saline vs. UT; ^ p =0.0192, IL-27 vs. UT. Sample sizes: P25: UT n =6, saline n =14, IL-27 n =14; P45: UT n =16, saline n =7, IL-27 n =9; P60: UT n =18, saline n =6, IL-27 n =5. B IL-27 injection resulted in more photoreceptor rows in the peripheral retina compared to saline injection controls at P45, indicating photoreceptor rescue. * p =0.001, n =3/groups. There was no difference in the number of photoreceptor rows between treatments in the central retina. C Representative images showing more photoreceptor nuclei in the outer retina in IL-27 injected eyes compared with saline controls in the peripheral (asterisks) but not the central retina (triangles). The DAPI signal brightness was increased for the central retina images while constructing the figure to better visualize the retinal layers. D IL-27 injection reduced the total number of dying photoreceptors in the ONL, measured by TUNEL assay as in , compared to saline * p =0.022, n =6 per group. E ERG analysis on IL-27 injected mice showed higher cone-driven responses than saline controls at the highest flash intensity at P45 but not other intensities, indicating a mild but significant improvement in visual function. Scotopic (white light, rod responses) and photopic (green light, cone responses) b-wave amplitudes for different flash intensities in log cd.s/m2 are shown. * p =0.045, averages and standard error are shown, IL-27 (IL) n =5, saline (S) n =8. F PNA-labeling to visualize cone photoreceptors shows higher numbers of cones in IL-27 injected eyes at P45 compared to saline * p =0.013, n =4 per group. G Representative images of PNA-labeled cone photoreceptors are shown (orange, asterisks). Scale bars, 25 µm. ONL, outer nuclear layer, INL, inner nuclear layer, GCL, ganglion cell layer

Article Snippet: The rd10 mice (B6.CXB1- Pde6b rd10 /J, stock number 004297) were purchased from Jackson Laboratory (Bar Harbor, ME).

Techniques: Injection, Saline, TUNEL Assay, Labeling

Journal: Cell Communication and Signaling : CCS

Article Title: Interleukin 27-induced photoreceptor survival is associated with suppression of a novel Muller glia subpopulation

doi: 10.1186/s12964-026-02885-1

Figure Lengend Snippet: Immunodetection of Tox2 and Agmo. ( A ) Representative IHC images of retinal cryosections showing Tox2 expression at P20 and P25 in saline- and IL-27-treated rd10 eyes. Higher immunodetection of Tox2 in a pattern consistent with Muller glia radial processes (arrow) is observed in IL-27 treated retinas compared with control at P25. ( B ) Representative IHC images of retinal cryosections showing Agmo expression at P20 and P25 in saline- and IL-27-treated rd10 eyes. Intense immunodetection was shown at P20 in both treatments with minimal staining at P25. Detection of Agmo in a pattern consistent with Muller glial radial processes is indicated by the arrows. ( C ) Immunodetection of Muller glia markers GFAP and glutamine synthetase (GS) to demonstrate the morphologies of the glial radial processes (arrows). Retinal layers are indicated as follows: ONL, outer nuclear layer, OPL, outer plexiform layer, IPL, inner plexiform layer, INL, inner nuclear layer, GCL, ganglion cell layer. Scale bars, 25 µm

Article Snippet: The rd10 mice (B6.CXB1- Pde6b rd10 /J, stock number 004297) were purchased from Jackson Laboratory (Bar Harbor, ME).

Techniques: Immunodetection, Expressing, Saline, Control, Staining

Journal: iScience

Article Title: Efficient derivation of hiPSC-derived photoreceptor precursor cells and their neuroprotective effects in retinal degeneration

doi: 10.1016/j.isci.2025.114196

Figure Lengend Snippet: Differentiation and characterization of hiPSCs into PPCs (A) Schematic representation of the three-stage chemically defined differentiation protocol for generating PPCs from hiPSCs. Stage 1 (day 0 to day 10): HiPSCs induction using Neural Induction Medium to generate NSCs. Stage 2 (day 10 to day 17): RPC specification via Neural Differentiation Medium. Stage 3 (day 17 to day 24): terminal differentiation into PPCs using Neural Differentiation Medium supplemented with AM580. (B) Flow cytometry analysis of PPCs derived from hiPSCs. Unstained cells served as the negative control. (C) CRX expression in PPCs, as determined by flow cytometry. (D) LHX4 expression in PPCs, as determined by flow cytometry. (E) Expression levels (transcripts per million, TPM) of photoreceptor markers (GNB1, PDE6B, and GNB3) and RGC markers (POU4F2 and SHH) in undifferentiated iPSCs versus PPC-derived populations harvested at day 54 of differentiation ( n = 3). Photoreceptor markers show robust induction in PPCs, while RGC markers remain undetectable. Data confirm progression toward advanced photoreceptor maturation, aligning with late retinogenesis trajectories. Data are presented as mean ± SEM.

Article Snippet: The experimental animals used in this study included male C57BL/6J (C57) WT mice (8 weeks old) purchased from Wuhan Zikeheng Biotechnology Co., Ltd., and male Pde6b rd10/rd10 (rd10) mice (5 weeks old) obtained from Cyagen Biosciences Inc.

Techniques: Flow Cytometry, Derivative Assay, Negative Control, Expressing

Journal: iScience

Article Title: Efficient derivation of hiPSC-derived photoreceptor precursor cells and their neuroprotective effects in retinal degeneration

doi: 10.1016/j.isci.2025.114196

Figure Lengend Snippet: Subretinal injection of hiPSC-derived PPCs in C57 and rd10 mice (A) Schematic of subretinal injection (SRI): Cell suspensions containing hiPSC-derived PPCs were transplanted into the murine SRS. (B) Representative OCT cross-section of sham surgery control (C57 + solvent) demonstrating intact laminar architecture with well-defined GCL, INL, and ONL. (C) OCT imaging of C57 recipient retina 3 months post-transplantation reveals stable subretinal localization of donor cell clusters, with preserved host retinal stratification and no signs of structural disruption. (D) OCT profile of sham surgery control (rd10 + solvent) showing advanced degenerative pathology, including ONL thinning. (E) Longitudinal OCT tracking of rd10 recipients at 90 days post-transplantation shows persistent localization of PPC-derived cellular aggregates in the SRS, with no evidence of retinal detachment. Scale bars represent 100 μm.

Article Snippet: The experimental animals used in this study included male C57BL/6J (C57) WT mice (8 weeks old) purchased from Wuhan Zikeheng Biotechnology Co., Ltd., and male Pde6b rd10/rd10 (rd10) mice (5 weeks old) obtained from Cyagen Biosciences Inc.

Techniques: Injection, Derivative Assay, Control, Solvent, Imaging, Transplantation Assay, Disruption

Journal: iScience

Article Title: Efficient derivation of hiPSC-derived photoreceptor precursor cells and their neuroprotective effects in retinal degeneration

doi: 10.1016/j.isci.2025.114196

Figure Lengend Snippet: Photoreceptor lineage commitment and presynaptic structure formation of hiPSC-derived PPCs in rd10 retinas (A) Glial and immune microenvironment characterization: analysis of activated Müller cell marker GFAP reveals localized Müller cell activation near the graft. (B) Metabolic viability assessment: hMito confirms abundant metabolically active donor cells within the grafted clusters. (C) Photoreceptor maturation: Robust expression of rod-specific RHO in donor cells demonstrates subtype-specific differentiation. Triangular arrows highlight RHO + /Ku80 + co-localization. (C′) No RHO + expression was detected in the SRS distal to the injection site of rd10 mice. (D) Immunostaining for presynaptic marker CtBP2 and BC marker PKCα shows CtBP2 + puncta located near PKCα + BC dendrites. Triangular arrows highlight areas of proximity. Scale bars represent 50 μm.

Article Snippet: The experimental animals used in this study included male C57BL/6J (C57) WT mice (8 weeks old) purchased from Wuhan Zikeheng Biotechnology Co., Ltd., and male Pde6b rd10/rd10 (rd10) mice (5 weeks old) obtained from Cyagen Biosciences Inc.

Techniques: Derivative Assay, Marker, Activation Assay, Metabolic Labelling, Expressing, Injection, Immunostaining

Journal: iScience

Article Title: Efficient derivation of hiPSC-derived photoreceptor precursor cells and their neuroprotective effects in retinal degeneration

doi: 10.1016/j.isci.2025.114196

Figure Lengend Snippet: Differentiation and characterization of hiPSCs into PPCs (A) Schematic representation of the three-stage chemically defined differentiation protocol for generating PPCs from hiPSCs. Stage 1 (day 0 to day 10): HiPSCs induction using Neural Induction Medium to generate NSCs. Stage 2 (day 10 to day 17): RPC specification via Neural Differentiation Medium. Stage 3 (day 17 to day 24): terminal differentiation into PPCs using Neural Differentiation Medium supplemented with AM580. (B) Flow cytometry analysis of PPCs derived from hiPSCs. Unstained cells served as the negative control. (C) CRX expression in PPCs, as determined by flow cytometry. (D) LHX4 expression in PPCs, as determined by flow cytometry. (E) Expression levels (transcripts per million, TPM) of photoreceptor markers (GNB1, PDE6B, and GNB3) and RGC markers (POU4F2 and SHH) in undifferentiated iPSCs versus PPC-derived populations harvested at day 54 of differentiation ( n = 3). Photoreceptor markers show robust induction in PPCs, while RGC markers remain undetectable. Data confirm progression toward advanced photoreceptor maturation, aligning with late retinogenesis trajectories. Data are presented as mean ± SEM.

Article Snippet: Pde6b rd10/rd10 mice , Cyagen Biosciences , Cat# C001276.

Techniques: Flow Cytometry, Derivative Assay, Negative Control, Expressing